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The captured troponin is then reacted using a second antibody and, in some assays, a 3rd antibody coupled to an indicator molecule.[36] The assays differ from each other by the kinds of antibody employed, by the epitopes to which they bind, and by the sort of indicator molecule that may be utilized.[46]
[fifty nine] Second, measured assay concentrations fall short being reliable mainly because cTnI circulates in several sorts; the antibodies Utilized in the out there assays recognize distinct epitopes of cTnI even for assays and devices promoted by the identical producer.[sixty]
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